U.S.-Korea Cooperative Research: Carbon Monoxide as a Substrate for Microbial Maintenance

美韩合作研究：一氧化碳作为微生物维持的底物

• 批准号: 0342779
• 负责人: Gary King
• 金额: --
• 依托单位: University of Maine
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 2004
• 资助国家: 美国
• 起止时间: 2004-08-01 至 2008-02-29
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=0342779&HistoricalAwards=false
• 关键词: U.S.; Korea; Cooperative; Research; Carbon

0342779KingBacteria play an important role in the global budget of carbon monoxide (CO). Largely unknown bacterial populations in soils and the water column of aquatic systems oxidize hundreds of teragrams per year, or about 10%-20% of the estimated annual flux to the atmosphere. In spite of their biogeochemical significance, relatively little is known about the identity of CO-oxidizing populations active in situ, their phylogenic and physiological diversity or the importance of CO as substrate for their basic metabolic needs. of CO oxidizers. It is clear that CO at high concentrations ( 1000 ppm) can serve as a sole source of cell carbon and energy for laboratory-grown cultures, but ambient CO concentrations seldom reach or exceed a few ppm. Bacteria readily use such concentrations, but are they sufficient to contribute significantly to cellular metabolic needs?This award supports Professor Gary M. King and one of his graduate students at the University of Maine to collaborate in research on this question with Dr. Y. M. Kim and his research group from Yonsei University in Korea. They will evaluate the significance of low, ecologically realistic CO concentrations for growth and survival of two common soil isolates, Mycobacterium smegmatis and Bradyrhizobium japonicum. This collaborative project will include both molecular and physiological analyses of the ability of CO at low concentrations to promote the survival and viability of CO oxidizers under conditions relevant to their natural dynamics in soils. Dr. Kim has extensive experience with the molecular biology, physiology and growth of CO-oxidizing bacteria, including mycobacteria, and characterization of the genetics and biochemistry of CO dehydrogenase, while Dr. King has extensive experience in studying the ecophysiology and biogeochemistry of microbial trace gas utilization, analytical techniques and instrumentation for near-ambient CO analyses, and expertise in the design of microcosms and gas flow systems for research. Broader significanceResults of the study will significantly improve our understanding of the ecophysiology of microbial CO metabolism, particularly at real-world levels. This information is an important factor in predicting potential responses to long-term, regional-scale disturbances in climate, land use and eutrophication of aquatic systems.The work will involve exchanges of both U.S. and Korean graduate students, who will benefit from learning new research techniques and from the cultural experience. The international research experience is likely to expand the future research opportunities of the US students in Dr. King's group. The exchange of techniques and methods in this research partnership will enhance the capabilities of both labs to address topical issues in the ecophysiology of microbial groups that affect atmospheric composition.

0342779Kingbacteria在一氧化碳（CO）的全球预算中起着重要作用。 土壤和水生系统的水柱在很大程度上未知的细菌种群每年氧化数百个Teragram，约为估计的大气估计通量的10％-20％。 尽管它们具有生物地球化学意义，但对现场的共同氧化种群的身份，系统发育和生理多样性或CO作为基本代谢需求的基础的重要性知之甚少。 CO氧化剂。 显然，高浓度（1000 ppm）的CO可以作为实验室生长培养物的细胞碳和能量的唯一来源，但是环境CO浓度很少达到或超过几ppm。 细菌很容易使用这种浓度，但是它们足以满足细胞代谢需求吗？该奖项支持Gary M. King教授及其缅因州大学的一位研究生，与Y. M. Kim博士及其Yonse Inosedical的研究小组合作就此问题进行了研究。他们将评估低生态现实的CO浓度对两个常见的土壤分离株，分枝杆菌和japonicum的生长和存活的重要性。 该协作项目将包括CO在低浓度下促进CO氧化剂在与土壤中自然动力学相关的条件下促进CO氧化剂生存能力和生存能力的能力的分子和生理分析。 金博士在共同氧化细菌的分子生物学，生理学和生长方面拥有丰富的经验，包括分枝杆菌，以及二氢酶的遗传学和生物化学的表征，而金博士在研究生态生理学和生态生理学和生物化学方面具有丰富的经验。用于研究的微观和气流系统。该研究的更广泛的显着性将显着提高我们对微生物CO代谢的生态生理学的理解，尤其是在现实世界中。 这些信息是预测对水生系统中气候，土地使用和富营养化的长期，区域尺度干扰的潜在反应的重要因素。这项工作将涉及美国和韩国研究生的交流，他们将从学习新的研究技术和文化经验中受益。 国际研究经验可能会扩大金博士小组中美国学生的未来研究机会。 这项研究伙伴关系中的技术和方法的交换将增强两个实验室的能力，以解决影响大气组成的微生物群体生态生理学中的局部问题。