Analysis of Centromere-Specific Genes in Yeast

这个ROW职业发展奖的长期目标是 确定真核染色体的动粒区域 与纺锤体纤维相连，以确保正确的染色体 种族隔离 最终，调查人员将调查 动粒在组织染色体中的作用 核在间期。 该项目将利用 基因和生物化学的方法。 抗体将 制备并用于确定蛋白质的细胞定位 对于各种突变细胞，并将有助于分离 与蛋白质相互作用的成分。 这些强大 方法将允许直接调查要求 用于酵母中的动粒组装和功能。 这种方法， 结合经典的遗传方法，使酵母成为一种 用于测定分子量的优良模型真核系统 细胞成功分裂的基本机制

The long-term goal of this ROW Career Advancement Award is to determine how the Kinetochore region of a eucaryotic chromosome associates with a spindle fiber to ensure proper chromosome segregation. Eventually, the investigator will investigate the role the kinetochore plays in organizing the chromosomes in the nucleus during interphase. This specific project will make use of both genetic and biochemical approaches. Antibodies will be prepared and used to determine the cellular location of proteins for various mutant cells and will facilitate the isolation of components with which the proteins interact. These powerful approaches will permit direct investigation of the requirements for kinetochore assembly and function in yeast. This approach, combined with classical genetic approaches, make yeast an excellent model eucaryotic system for determining the molecular mechanisms underlying successful cell division.