Research Initiation: High Density Cultivation of Bacillus Thuringiensis Cells

这项研究将集中在实现高生产率的 .-苏云金芽孢杆菌细胞内毒素蛋白的研究 搅拌生物反应器的分批操作。 该战略涉及增长 在营养生长期， 将控制发酵参数以最小化 有害的有毒产品。 最低营养需求 细胞生长到高密度将通过连续 功法 将对补料分批培养进行微调 因此高密度介质组合物对于随后的 孢子形成和内毒素形成。 生物杀虫剂越来越被视为 比通常是能量的化学杀虫剂更有利， 密集，半衰期长，对许多无害的 生命的形式。 .-内毒素是一种商业化生产的杀虫剂 这是一个正在广泛研究的问题， 力量 此外，孢子形成杆菌通常用于 生产许多工业上重要的产品;因此， 涉及高密度培养的研究可以提高 几个过程。

This study will concentrate upon achieving high productivity of .-endotoxin protein by Bacillus Thuringiensis cells by controlled fed- batch operation of stirred bioreactors. The strategy involves growing the cells to high concentrations in vegetative phase during which the fermentation parameters will be controlled to minimize formation of undesirable toxic products. Minimal requirements of nutrients for cell growth to high densities will be established by continuous cultivation techniques. The fed-batch cultivation will be fine tuned so that medium composition at high densities is optimum for subsequent sporulation and endotoxin formation. Biological insecticides are increasingly being viewed as more advantageous than chemical pesticides which are generally energy intensive, have long half lives, and adversely affect many harmless forms of life. .-endotoxin is a commercially produced insecticide that is being studied extensively with major improvements in its potency. In addition, spore-forming bacilli are commonly used to produce many industrially important products; therefore, fundamental studies involving high density cultivation can improve productivity of several processes.