Phenotypic Plasticity in Neural Crest Development

这些实验将探索神经递质的可塑性， 胆碱能神经元 这项研究的长期目标是 了解细胞谱系的决定， 神经嵴细胞分化 初步结果显示， 结果显示，来自睫状神经节的胆碱能神经元 当移植到 躯干体节 这项研究将进一步扩大和 描述这一观察结果，并将检查表型 其他类型神经元的可塑性。 实验范例 利用新的细胞标记技术， 逆行运输荧光素乳胶的神经元 微球 标记后，神经元将被 显微注射到不同区域的神经嵴通路上， 胚胎使用的注射技术， 实验室 通过这种方式，可以将细胞引入到 无论是正常或异位部位在各种发育 阶段

These experiments will probe neurotransmitter plasticity in cholinergic neurons. The longterm goals of this research are to understand the cell lineage decisions involved in aspects of neural crest cell differentiation. The preliminary results have shown that cholinergic neurons derived from the ciliary ganglion can acquire adrenergic properties when transplanted into the trunk somites. This research will extend and further characterize this observation and will examine the phenotypic plasticity of other types of neurons. The experimental paradigm utilizes a new cell marking technique for selectively labeling neurons by retrograde transport of flourescent latex microspheres. After labeling, the neurons will then be microinjected onto neural crest pathways in different regions of the embryo using an injection technique developed in this laboratory. In this way, it is possible to introduce cells into either normal or ectopic sites at a variety of developmental stages.