RUI: Transport in Polyelectrolyte Solutions

在高浓度下聚合物运动的性质是 目前的科学兴趣。 带电粒子的扩散 （“聚合物”）通过其他聚合物的溶液 发生在几种情况下，包括细胞内和细胞间 蛋白质转运和用于分离重组体的方法 DNA 研究人员将进行系统的实验 不同条件下聚电解质-聚合物互扩散研究 条件 光散射光谱将用于研究扩散 聚苯乙烯磺酸盐，无论是在简单的解决方案和在 第二（“基质”）聚合物的存在。 基体聚合物和 溶剂将不散射光，因为它们是等折射。 要系统地改变的实验变量包括 - 两种聚合物的浓度和分子量，以及 溶液的离子强度 通过测量极化以及 去极化光谱，探针的自扩散系数为 以及它的旋转扩散系数。 这些将与现有模型的预测进行比较 聚合物动力学，从而测试这些模型，并澄清 了解聚合物如何在溶液中移动。

The nature of polymer motion at elevated concentration is a matter of current scientific interest. The diffusion of charged ("polyelectrolyte") polymers through solutions of other polymers occurs in several contexts, including intra-and intercellular protein transport and in methods for isolation of recombinant DNAs. The investigators will undertake a systematic experimental study of polyelectrolyte-polymer interdiffusion under different conditions. Light-scattering spectroscopy will be used to study diffusion of polystyrene sulfonate, both in simple solution and in the presence of a second ("matrix") polymer. The matrix polymer and solvent will scatter no light because they will be isorefractive. Experimental variables to be varied systematically include the concentrations and molecular weights of the two polymers, and solution's ionic strength. By measuring polarized as well as depolarized spectra, the probe's self-diffusion coefficient as well as its rotational diffusion coefficient will be obtained. These will be compared with predictions of the available models of polymer dynamics, thereby testing these models and clarifying understanding of how polymers move in solution.