Mobility of Proteins Adsorbed at Solid-Liquid Interfaces

这项工作的主要目标是表征流动性的 吸附在固液界面的蛋白质。 本研究 代表了蛋白质吸附研究重点的转移 对吸附的复杂行为的分子理解 proteins. 希望量化吸附蛋白质的迁移率， 描述影响流动的因素，确定流动的机制， 吸附的蛋白质翻译，并测量自组装的程度 蛋白质转化为表面岛屿。 使用总计 内反射荧光和荧光恢复后 图案光漂白，该研究将确定蛋白质表面 扩散系数和吸附的蛋白质的分数， 移动的。 流动性将被量化为剪切速率的函数， 表面覆盖率和表面停留时间，以确定 支配因素 了解吸附蛋白质的流动性，以及 蛋白质结构和组织的相关问题将有助于 开发生产蛋白质的有效方法， 多肽材料。 这些材料的用途正在增加， 不仅作为药物，而且作为一种新的有机方法， 催化，并作为化学生物传感器中的活性剂 分析. 蛋白质或多肽吸附到固体表面， 不可避免地在所有过程中制造，修改，或 净化这些物质。 这对蛋白质尤为重要 和多肽的制备，其中吸附的蛋白质层可以 影响其接触的细胞的生物活性， 包括基因工程微生物， 受雇于工业。 此外，A的适当功能 蛋白质或多肽的保存取决于正确的 结构 吸附到固体表面可以改变这一点 结构

The primary objective of this work is to characterize the mobility of proteins adsorbed at solid-liquid interfaces. This research represents a shift in the emphasis of protein adsorption studies towards a molecular understanding of the complex behavior of adsorbed proteins. It is hoped to quantify the mobility of adsorbed proteins, characterize the factors governing mobility, identify the mechanism of adsorbed protein translation, and measure the extent of self-assembly of proteins into surface islands. Using a combination of total internal reflection fluorescence and fluorescence recovery after pattern photobleaching, the research will determine protein surface diffusion coefficients and the fraction of adsorbed proteins that are mobile. Mobility will be quantified as a function of shear rate, surface coverage, and surface residence time in order to identify the governing factors. Understanding the mobility of adsorbed proteins, as well as the related issues of protein structure and organization will aid the development of effective processes to manufacture protein and polypeptide materials. The utility of these materials is increasing, not only as pharmaceuticals, but also as novel means of organic catalysis, and as the active agent in biosensors for chemical analysis. Protein or polypeptide adsorption to solid surfaces is inevitable in all processes to manufacture, modify, or purify these materials. This is particularly important to protein and polypeptide manufacture in that an adsorbed protein layer can influence the biological activity of the cells it contacts, including the genetically engineered microorganisms commonly employed in industry. In addition, the proper function of a protein or polypeptide depends on the preservation of the correct structure. Adsorption to a solid surface can alter that structure.