Analytical Studies of Binding Protein Interactions with Enzyme-Ligand Conjugates

这个项目是在分析和表面化学的一般领域和生物分析化学的子领域。这里计划的研究集中在三个相关的研究领域。这三个领域包括：1)使用简单的均质方法开发选定蛋白质和除草剂的分析方法；2)使用中空透析纤维开发连续和可逆的生物分子检测方案；3)基础实验，旨在选择具有理想结合特性但很少或没有桥接基团识别的单克隆抗配体抗体。这里计划的研究旨在开发基于某些蛋白质的选择性相互作用的分析。这些检测应该具有几乎相同的选择性，但比基于使用抗体的检测便宜得多。此外，这种新方法不应要求使用放射性标签。

This project is in the general area of Analytical and Surface Chemistry and in the subfield of bioanalytical chemistry. The studies planned here focus on three related areas of research. These three areas include 1) developing analytical procedures for selected proteins and herbicides using simple homogeneous methodology, 2) the use of hollow dialysis fibers to develop a continuous and reversible biomolecule detection scheme, 3) fundamental experiments designed to select monoclonal anti-ligand antibodies with desirable binding characteristics but little or no bridging group recognition. The studies planned here are designed to develop assays based on the selective interactions of certain proteins. These assays should be almost as selective, but much less expensive than assays based on the use of antibodies. In addition, this new methodology should not require the use of radioactive labels.