Mechanisms of Virus-Mediated Translational Inhibition

人们正在尝试确定青蛙病毒3选择性阻断宿主细胞转译的机制。为了实现这一目标，计划鉴定和分离FV3病毒粒子相关的翻译抑制剂，通过鉴定起始翻译缺陷发生的位置来确定FV3介导的翻译关闭机制。并确定病毒信息的5'非翻译区是否控制它们的翻译效率。在过去的二十年中，真核生物所涉及的事件序列和特定成分已经确定，翻译控制在基因表达中的重要性已经得到普遍认可。然而，尽管这两个领域都取得了进展，但仍存在重大差距。例如，几种真核起始因子的确切作用及其各种亚基的功能尚不清楚。鉴于此，很明显，模型系统（FV3就是其中之一）在促进我们对翻译和翻译控制的理解方面将是最重要的。Chinchar博士完全有资格进行这项研究。

Attempts are being made to determine the mechanism by which frog virus 3 selectively blocks host cell translation. To accomplish this it is planned to identify and isolate the FV3 virion-associated translational inhibitor, to determine the mechanism of FV3 mediated translational shut-off by identifying where in initiation the translational defect occurs, and to determine whether the 5' untranslated region of viral messages controls their translational efficiency. During the past twenty years, the sequence of events and specific components involved in eukaryotes have been determined and the importance of translation control in gene expression has gained general accpetance. However, despite advances in both areas, there remain significant gaps. For example the precise roles for several eukaryotic initiation factors and function(s) of their various subunits is not known. In light of this, it should be apparent that model systems, of which FV3 is one, will be most important in advancing our understanding of translation and translational control. Dr. Chinchar is well qualified to carry out this research.