Chloroplast-Derived Nuclear DNA: Prevalance and Mechanisms of Transposition

我们最近发现了叶绿体衍生的DNA序列 在我们克隆的番茄核基因的一个内含子中。 尽管已经证明，通过杂交 许多植物的细胞核基因组含有 从叶绿体基因组中获得的序列，这是 这种集成的物理隔离的第一个例子。 我们对整合者和整合地点的分析 暗示了同源重组的参与 转座事件中的机制。 在本提案中，我们 概述旨在物理隔离和 描述了番茄中许多叶绿体DNA整合体的特征 核基因组 获得的数据将用于测试 假设同源重组参与了 将这种叶绿体序列转座到核中 基因组 整合到细胞中的DNA序列的存在 起源于细胞器基因组的基因组已经被 大量记录在案 然而，我们所知甚少或一无所知 关于所涉及的机制。 本研究结果 应该能让我们深入了解这些过程

We have recently identified chloroplast-derived DNA sequences in an intron of a tomato nuclear gene that we had cloned. Although it had been demonstrated, by hybridization experiments, that the nuclear genomes of many plants contain sequences derived from the chloroplast genome, this is the first example of the physical isolation of such integrates. Our analysis of the integrates and of the integration sites suggests the involvement of homologous recombination mechanisms in the transposition events. In this proposal we outline experiments designed to physically isolate and characterize many chloroplast DNA integrates in the tomato nuclear genome. The date obtained will be used to test the hypothesis that homologous recombination is involved in the transposition of such chloroplast sequences into the nuclear genome. The presence of DNA sequences integrated into the cellular genome which originated in the organelle genomes has been abundantly documented. However, little or nothing is known about the mechanism(s) involved. The results of this study should provide insight into those processes.