Presidential Young Investigator Award: Modified and Linked Metalloproteins

这项由无机化学、生物无机化学和有机金属化学项目颁发的总统青年研究人员奖是在生物无机化学领域。金属离子将被用于多种方式来探测生物分子的结构和功能。非侵入性的过渡金属探针将通过选择性标记来绘制金属蛋白的结合表面，并将研究辅助配体对金属络合物与生物大分子选择性识别的影响。通过系统地研究金属蛋白活性中心与无机发色团的激发态之间的电子转移反应动力学和机理，探索金属蛋白中蛋白质内的电子转移。单核和多核过渡金属络合物将用于交联金属蛋白，以制备潜在的多电子氧化还原催化剂。将探索金属水合物对蛋白质和多肽的裂解，特别是将水解剂选择性地输送到一般酶不能裂解的多肽键上。最后，铂-195核磁共振谱将成为立体动力学研究的一种新技术。该项目有望为研究金属蛋白的功能提供通用的新工具。

This Presidential Young Investigator Award from the Inorganic, Bioinorganic and Organometallic Chemistry Program is in the area of bioinorganic chemistry. Metal ions will be used in a variety of ways to probe the structure and function of biomolecules. Noninvasive transition-metal probes will be used to map the binding surfaces of metalloproteins by selective tagging, and the effects of ancillary ligands on the selective recognition between metal complexes and biological macromolecules will be investigated. Intraprotein electron transfer in metalloproteins will be explored through a systematic study of the kinetics and mechanism of electron transfer reactions between active sites of metalloproteins and excited states of inorganic chromophores attached selectively to the protein backbone. Mononuclear and polynuclear transition metal complexes will be used to cross-link metalloproteins to produce potential multielectron redox catalysts. The cleavage of proteins and peptides by metal aquo complexes will be explored, with special focus on the selective delivery of hydrolytic agents to peptide bonds which are not cleavable by common enzymes. Finally, Pt-195 NMR spectroscopy will be developed as a new technique for the study of stereodynamics. This project is expected to provide versatile new tools for the investigation of the function of metalloproteins.