IUC Project: Adjustable Micelles in Supercritical Fluids

基于最近的工作，反胶束增溶亲水 超临界流体中反胶束的研究进展 流体，假设亲水性蛋白质可以容易地 在超临界流体中被反胶束吸收并从反胶束中沉淀 液 由于反胶束的聚集数变化很大， 其中有机物的溶解度参数用作溶剂， 由于超临界溶剂的溶解度参数变化 系统在相空间中的位置在 临界点时，反胶束的聚集数应变化 在超临界溶剂中，随着压力和温度的变化， 由于蛋白质在反胶束中的分配系数为 强烈依赖于反胶束的大小，轻微的变化 在高于临界点的条件下， 从反胶束进入水相。 这种现象，如果可以证明，将构成一个基础， 潜在有价值的生物分子分离方法。 该系统 有许多因素使它在以下方面非常有前途： 生物分离领域的工业应用。

Based on recent work on reverse micellar solubilization of hydrophilic proteins and recent observations of reverse micelles in supercritical fluids, it is hypothesized that hydrophilic proteins may be readily taken up by and precipitated from reverse micelles in a supercritical fluid. Since aggregation numbers of reverse micelles vary tremendously with the solubility parameter of the organic used as a solvent and since the solubility parameter of a supercritical solvent varies dramatically with the location of the system in phase space above the critical point, aggregation numbers of reverse micelles should vary dramatically with pressure and temperature in a supercritical solvent. Since the partition coefficient of proteins in reverse micelles is strongly dependent on the size of the reverse micelles, slight changes in conditions above the critical point might readily force the proteins from the reverse micelles into an aqueous phase. This phenomenon, if it can be demonstrated, would form the basis of a potentially valuable separation process for biomolecules. This system has many elements which make it highly promising with regard to industrial applications in the area of bioseparations.