Developing Adsorptive Protein Separations

这项研究将加快蛋白质吸附分离的设计， 重点是有效扩大规模。 重点是工具性的 直接测量关键分离参数的技术： O 颗粒内扩散系数，需要预测 任何吸附过程的有效性，特别是 这在商业分离器的高负载下是重要的。 脉冲场梯度核磁共振技术将用于此 目的. O 吸附动力学，需要预测分离度和产率 损失，并且在高溶质负载下再次最重要。 O 对流扩散系数，以表征 系统几何形状和流动条件的影响。 磁 将使用共振成像。 成功将允许基于基础运输的合理设计， 反应原理，从而不仅优化工艺条件， 而且还考虑替代的处理模式和设备 配置. 这项研究将促进治疗性蛋白质的开发， 其他重要的新药，并降低生产成本 通过提供用于下游处理，分离， 从发酵液中提纯 或其他原油来源。 下游加工占50至 占总生产成本的80%，但往往是 相对于其他方面， 生产过程 这里的注意力集中在吸附 分离，如色谱法，因为这些是 对两类至关重要的 治疗剂：蛋白质和合成有机物。 超速 如果我们要防止 竞争对手无法利用美国的发现 医学和生物学研究人员。

This study will speed the design of adsorptive protein separations, with emphasis on efficient scale-up. The focus is on instrumental techniques for direct measurement of key separation parameters: o Intraparticle diffusion coefficients, needed to predict effectiveness of any adsorptive process and especially important at the high loadings of commercial separators. Pulsed field gradient NMR techniques will be used for this purpose. o Adsorption kinetics, needed to predict resolution and yield losses, and again most important at high solute loading. o Convective dispersion coefficients, to characterize the effects of system geometry and flow conditions. Magnetic resonance imaging will be used. Success will permit rational design based on underlying transport and reaction principles, and thus not only to optimize process conditions but also to consider alternate processing modes and equipment configurations. This research will enhance the development of therapeutic proteins and other important new drugs and also reduce the cost of producing them by providing improved procedures for downstream processing, isolation, and purification from fermentation broths or other crude sources. Downstream processing accounts for 50 to 80 percent of total production costs but has tended to be neglected by engineers relative to other aspects of the production process. Attention is focused here on adsorptive separations, such as chromatography, because these are particularly effective for two critically important classes of therapeutic agents: proteins and synthetic organics. Speeding commercial development is necessary if we are to prevent competitors from capitalizing on the discoveries of American medical and biological researchers.