Pharmaceutical Production by Immobilized Cells

摘要：本提案支持康涅狄格大学Robert W. Coughlin博士和dr。埃及扎加齐格扎加齐格大学的A. Halim M.M. El-Sayed和Wafaa M. Mahmoud。每位埃及科学家都是和平奖学金的持有人，这将使他们能够在康涅狄格大学与Coughlin博士一起工作大约五个月。项目资金将允许(1)延长他们的停留时间，如果用于他们的研究进展，(2)允许他们返回埃及工作，然后第二次返回康涅狄格州继续项目工作，(3)使私人侦探能够访问扎加齐格大学，与他们一起工作第三期。这项合作利用固定化微生物细胞生产右旋糖酐（一种血浆扩张剂）和麻黄碱（一种广泛使用的治疗药物）。研究新的固定化方法、新的固定化载体和新的生产工艺。El-Sayed博士致力于从蔗糖中生产葡聚糖，而Mahmoud博士将致力于生产治疗药物l-麻黄碱。这两位埃及科学家提出的研究努力将把生产两种重要药物的技术引入埃及，并可能导致该国发展这两种药物的生产，从而使埃及人民受益，否则他们可能无法获得这些有效药物。范围：本提案将汇集两国大学在微生物转化和酶和细胞固定化领域的科研科学家。科学技术和研究方法的发展将从美国转移到埃及，首先是扎加齐格大学，如果成功，然后是埃及当地生产的两种重要药物，一种是血浆扩张剂，另一种是治疗药物。这项研究将需要开发新的通用知识和程序来固定细胞，设计生物催化剂和相关的生物反应器。该提案实现了发展中国家科学项目通过国际合作交流和转移科学知识的目标。

ABSTRACT NARRATIVE Description: This proposal supports the collaboration of Dr. Robert W. Coughlin, University of Connecticut and Drs. A. Halim M.M. El-Sayed and Wafaa M. Mahmoud of Zagazig University, Zagazig, Egypt. Each Egyptian scientist is a holder of a Peace Fellowship, which will enable them to spend about five months at the University of Connecticut working with the Dr. Coughlin. Project funding will allow (1) an extension of their stay, if appropriated for the progress of their research, (2) permit them to return to Egypt to work and then to return to Connecticut a second time to continue project work and (3) enable the P.I. to visit Zagazig University to work with them a third period. The collaboration is using immobilized microbial cells for producing dextran, a blood plasma expander, and ephedrine, a widely used therapeutic drug. New immobilization method, new immobilization carriers and new production technologies are being investigated. Dr El-Sayed is working on production of dextran from sucrose, while Dr. Mahmoud will work on production of the therapeutic drug, L-ephedrine. The proposed research efforts of the two Egyptian scientists will introduce technology for the production of two important drugs into Egypt and could lead to the development of their production in that country to benefit the Egyptian population that might not otherwise have access to these efficacious drugs. Scope: This proposal will bring together scientific research scientists from the universities of two countries working in the area of microbial transformations and immobilization of enzymes and cells. The development of scientific skills and research methods will be translated from the U.S. to Egypt, first to Zagazig University and if successful, then to local Egyptian production of two important drugs, one a plasma expander and the second, a therapeutic. This research will require the development of new general knowledge and procedures for immobilizing cells and designing biocatalysts and associated bioreactors. This proposal fulfills objectives of the Science in Developing Countries Program in its exchange and transfer of scientific knowledge through a international collaboration.