Lignin Degradation by Phanerochaete Chrysosporium: Biochemical Characterization of Extracellular Peroxidases

本项目的总体目标是表征两种酶，木质素过氧化物酶（LiP）和锰过氧化物酶（MnP）在白腐菌黄孢Phanerochaete chrysosporium氧化降解木质素中的生化功能。将进行动力学、光谱和反应性实验，以确定戊曲醇在LiP的机制和调控中的作用。稳态动力学测量、EPR和共振拉曼光谱将用于表征MnP的活性中心和Mn在酶中作为氧化还原偶对的作用。木质素是地球上第二丰富的生物高聚物，也是最丰富的天然芳香物质。了解这些酶在木质素生物降解中的作用可能会导致许多有用的生物技术应用的发展，例如在纸浆造纸和化学工业中的应用。与现有工艺相比，它们对能源的需求更少，也更环保。

The overall objective of this project is to characterize the biochemical function of two enzymes, a lignin peroxidase (LiP) and a manganese peroxidase (MnP), in the oxidative degradation of lignin by the white-rot fungus Phanerochaete chrysosporium. Kinetic, spectroscopic and reactivity experiments will be conducted to ascertain the role of veratryl alcohol in the mechanism and regulation of LiP. Steady-state kinetic measurements, EPR, and resonance Raman spectroscopy will be used to characterize the active center of MnP and the role of Mn as redox couple in the enzyme. Lignin is the second most abundant biopolymer and the most abundant natural aromatic material on earth. An understanding of the role of these enzymes in the biodegradation of lignin may lead to the development of many useful biotechnological applications such as those in the pulp-paper and chemical industries. They would be less energy demanindg and more environmentally benign than existing processes.