Molecular Control of Ammonia Assimilation in Alfalfa Root Nodules

本研究的目的是描述 氮同化的生物化学和分子生物学 苜蓿（Medicago sativa）与细菌共生 苜蓿根瘤菌，并确定其对植物寄主的影响 在调节根瘤形成和固氮中的作用。 的 拟议中的研究将测试这一假设，即同化的 在固氮过程中产生的氨涉及 根瘤的表达。氮素酶的特殊形式 同化途径，并将通过分析 磷酸烯醇丙酮酸羧化酶基因的表达， 苜蓿中的谷氨酸合酶和天冬氨酸氨基转移酶 固氮菌和非固氮菌 结节 这项研究将增加对植物寄主的认识 对固氮的贡献。 它有可能 植物与细菌基因协同作用机制研究 在农业和经济上参与这一表达 重要的过程，并间接地提高其效率。

The objective of this research is to characterize the biochemistry and molecular biology of nitrogen assimilation of alfalfa (Medicago sativa) in symbiosis with the bacterium Rhizobium meliloti, and to determine the effect of the plant host in regulation of nodule formation and nitrogen fixation. The proposed research will test the hypothesis that assimilation of ammonia produced during nitrogen fixation involves the the expression of nodule.specific forms of enzymes of the nitrogen assimilation pathway, and will be pursued through analysis of the expression of genes for phosphoenolpyruvate carboxylase, glutamate synthase, and aspartate amino transferase in alfalfa strains that form nitrogen fixing and non.nitrogen fixing nodules. This research will increase knowledge of plant host contributions to nitrogen fixation. It has the potential to identify mechanisms of coordination of plant and bacterial gene expression involved in this agronomically and economically important process and, indirectly, to increase its efficiency.