Modeling and Measurement of Convection and Diffusion in Immobilized Cell Systems

本研究的目标是数学建模和测量两种固定化细胞系统的质量传输：中空纤维生物反应器和多孔基质（头和板结构）。这需要分别研究填充细胞床中的扩散和对流行为，使用磁共振成像（MRI）测量无人（无细胞）中空纤维和多孔珠系统中的对流速度，以及MRI测量有（哺乳动物细胞）系统中的流体输送。这些信息将提供与生物反应器设计、操作和放大相关的问题的答案。

The goals of this research are to mathematically model and measure mass transport in two immobilized cell systems: a hollow fiber bioreactor and a porous matrix (bead and slab configurations). This entails separate investigations of the behavior of diffusion and convection in packed cell beds, measurement of convective velocities in unpopulated (no cells) hollow fiber and porous bead systems using magnetic resonance imaging (MRI), and MRI measurement of fluid transport in the populated (with mammalian cells) systems. It is intended that this information will provide answers to questions of relevance to bioreactor design, operation, and scale-up.