Genetic Analysis of the t-Complex

先前的工作表明，野生鼠的许多完整的t单倍型和许多单倍型是t特有的等位基因和野生型等位基因的组合。这些特殊的等位基因被限制在t复合体的远端部分，这表明染色体重排的性质以及重组的结果在复合体的近端和远端部分是不同的。所提出的研究的第一个目标包括检验提出的解释TO复合体起源和进化的假说的几个方面，通过对所选等位基因的限制片段和序列分析来估计完整的T单倍型、马赛克单倍型及其野生型同源物的近端和远端部分的相对年龄，测试异常位点是否局限于t复合体的远端部分，鉴定野生小鼠的部分t单倍型，确定导致马赛克单倍型的重组事件的边界，并估计欧洲Mus亚种之间的基因流和导入速率。第二个目标涉及对t复合体内存在一个以上尾部相互作用(TCT)位点的假设的基因定位测试，以及对其中一个TCT位点可能所在的35kb片段的分子分析。家鼠体内的t复合体为研究基因复合体的起源和进化提供了独特的机会，特别是那些通过优先传播自己而对宿主不利的遗传寄生虫。

Previous work has shown that many complete t haplotypes and many haplotypes in wild mice are combinations of t-specific and wild-type alleles. These exceptional alleles were confined to the distal portion of the t complex, suggesting that the nature of the chromosomal rearrangements and therefore the consequences of recombination differ between the proximal and distal portions of the complex. The first goal of the proposed studies involves testing several aspects of a hypothesis proposed to account for the origin and evolution of the to complex, by estimating the relative ages of the proximal and distal portions of the complete t haplotypes, mosaic haplotypes and their wild-type homologues through restriction fragment and sequence analysis of selected alleles, testing whether exceptional loci are confined to the distal portion of the t complex, characterizing partial t haplotypes in wild mice, identifying the boundaries of the recombination events leading to mosaic haplotypes, and estimating rates of gene flow and introgression between subspecies of Mus in Europe. The second goal involves a gene mapping test of the hypothesis that there is more than one tail interaction (tct) locus within the t complex and molecular analysis of a 35kb segment where one of the tct loci is presumably located. The t complex in house mice offers unique opportunities for studying the origin and evolution of gene complexes, especially those that act as genetic parasites by preferentially transmitting themselves to the presumed detriment of the host.