DNA Replication in Bacteria: Control of Initiation

我们研究的长期目标是了解 细胞将DNA复制与细胞生长偶联， 分子基础和机制参与控制 细菌中DNA复制的起始速率。 我们有 最近发现，启动蛋白，DnaA， 和起始增强子mioC启动子的活性， 生长速率调节;这些调节的分子基础 过程将被确定。 我们将研究RNA是否 转录实际上是所有启动事件所必需的， 仅在从慢速到快速转换时由细胞使用 使用遗传学和生物化学的方法。 增长 将确定启动的特征和时间， 具有转录终止和远程稳定性的突变体 在oriC附近引入抑制信号。 这些研究将 为mioC启动子区的作用提供了明确的证据 和转录在调节起始事件和 将细胞生长与基因组复制相结合。

The long term goals of our research are to understand how the cell couples DNA replication with cell growth and to determine the molecular basis and mechanisms involved in controlling the rate of initiation of DNA replication in bacteria. We have recently discovered that levels of the initiator protein, DnaA, and activity of the initiation enhancer, the mioC promoter, are growth rate regulated; the molecular basis of these regulatory processes will be determined. We will examine whether RNA transcription is actually required for all initiation events or only used by the cell when making transitions from slow to fast growth rates using genetic and biochemical approaches. Growth characteristics and timing of initiation will be determined for mutants with transcription termination and telestability inhibitory signals introduced near oriC. These studies will provide definitive evidence for roles of the mioC promoter region and transcription in the regulation of initiation events and in coupling cell growth to genome duplication.