Identification of Sequences Necessary for the Regulation and Function of p33 in Carrot

植物细胞壁中的伸展蛋白mRNA和蛋白质已被证明是 增加对创伤乙烯病原体的反应 感染和暴露于真菌和植物细胞壁 激发子，这表明该细胞具有保护功能 壁蛋白 这个项目研究另一种细胞壁蛋白 在胡萝卜贮藏根和大豆中，称为p33， 在受伤组织中增加，并且在正常组织中也起作用。 植物发育 最近发现了一些 脯氨酸/羟脯氨酸丰富的蛋白质基因， 与胡萝卜p33序列的序列同源性表明， p33可能代表一类新的细胞壁结构 proteins. 启动子缺失分析用于鉴定DNA 序列负责合成p33， 受伤的胡萝卜根 这些研究将使 调节p33表达的顺式作用区域在 受伤的组织，并允许确定这些相同的 序列用于调节p33的表达， 正常的植物发育。 特异性缺失分析 胡萝卜p33序列的区域将用于鉴定 p33蛋白中可能的“结构域”， 功能和与其他细胞壁成分的相互作用。 这些结构域缺失研究将代表一种新的方法 涉及鉴定可能是生物学中 高等植物细胞壁结构蛋白的功能 植物。% 高等植物的细胞壁是动态结构， 在组成和结构上发生变化， 细胞年龄、组织类型和环境刺激。 大部分 细胞壁结构蛋白成分分析 研究了伸展蛋白，一种富含羟脯氨酸的糖蛋白， 以及编码它的基因。这项研究集中在一个新的 发现了细胞壁蛋白， 植物生长.*//

Extensin mRNA and protein in plant cell walls have been shown to increase in response to wounding, ethylene, pathogen infection and exposure to fungal and plant cell wall elicitors, suggesting a protective function for this cell wall protein. This project studies another cell wall protein in carrot storage roots and soybean, called p33, that also increases in wounded tissue and also plays a role in normal plant development. The recent identification of a number of proline/hydroxyproline-rich protein genes which share sequence homology with the carrot p33 sequence suggests that p33 may represent a new class of structural cell wall proteins. Promoter deletion analysis is used to identify DNA sequences which are responsible for the synthesis of p33 in wounded carrot roots. These studies will allow the cis-acting regions which modulate p33 expression in wounded tissue and allow determination of whether these same sequences are used to regulate the expression of p33 during normal plant development. Deletion analysis of specific regions of the carrot p33 sequence will be used to identify possible "domains" within the p33 protein necessary for its function and interaction with other cell wall components. These domain deletion studies will represent a new approach to identifying sequences which may be necessary for the function of structural cell wall proteins in higher plants.%%% The cell walls of higher plants are dynamic structures which undergo changes in composition and structure in relation to cell age, tissue type and environmental stimuli. Much of the analysis of the structural protein component of the cell wall has dealt with extensin, a hydroxyproline-rich glycoprotein, and the genes which encode it. This study centers on a newly discovered cell wall protein which plays a role in normal plant growth.***//