Microfluorometric and ENG'G Analyses of Immobilized Recom- binant Cell Systems

基因工程细胞有望用于生产 一系列生物学上重要的化合物 相对温和和可控的生物反应器条件。 基因工程细胞的固定化 在工业中被认为是更频繁的，因为这 配置允许高浓度的存在， 生物催化剂（细胞），从而保持 保证高体积生产率。 的 拟议中的研究将采用扫描技术， 固定化细胞群的显微荧光法 第一次回答以下问题： 固定化是否增加重组质粒的稳定性 在下一代？ 固定是否影响 质粒拷贝数、产物形成率和细胞 新陈代谢？ 测量的生物速率是否可以 描述了一个结构化的 重组细胞生化工程模型 新陈代谢，以前由一个 恒化器和其他制服调查员 环境？ 一种新型的高分辨率荧光显微镜将是 用于检测基因工程细菌细胞 生长到非常高的浓度。 结果是 研究将允许设计更有效的反应堆， 利用许多基本的发现， 新的生物技术产生的。 本 工程研究将有助于 基于这些新颖的方法的商业化 生物系统，通过提供定量观察 在商业上可行的条件下。

Genetically engineered cells hold promise for production of a wide range of biologically important compounds under relatively mild and controllable bioreactor conditions. The immobilization of genetically engineered cells is now being considered more frequently in industry, as this configuration allows the presence of a high concentration of the biological catalyst (the cells), and thus holds promise for high volumetric production rates. The proposed research will apply the technique of scanning microfluorimetry to immobilized cell populations for the first time in order to answer the following questions: Does immobilization increase recombinant plasmid stability in succeeding generations? Does immobilization influence plasmid copy number, product formation rates, and cell metabolism? Can the measured biological rates be described with an extended version of a structured biochemical engineering model for recombinant cell metabolism, previously developed by one of the investigators for chemostat and other uniform environments? A novel high resolution fluorescence microscope will be used to examine genetically engineered bacterial cells grown to very high concentrations. The results from this research will allow design of more efficient reactors to take advantage of the many fundamental discoveries which the new biotechnology has produced. The present engineering research will assist the pace of commercialization of processes based on these novel biological systems, by providing quantitative observation of these cells under commercially realistic conditions.