Regulation and function of yeast transcr#itional activator ADR1

本研究的目的是阐明 真核基因调控 选择进行研究的系统是 酵母转录激活因子ADR 1对葡萄糖的调控 抑制性醇脱氢酶（ADH 2）。 特别强调 将被放置在定义ADR 1的机制， 调节，ADR 1的功能区域， 介导或影响ADR 1功能，以及这些功能的方式 ADH 2转录受ADH 2转录因子和ADR的调控。 的组合 将使用生物化学、重组DNA和遗传分析。 将鉴定ADR 1的体内磷酸化位点， cAMP依赖性（cAPK）和非依赖性蛋白作用 激酶在ADR 1调节中的作用将被确定。 的机制 ADR 1将被识别。 ADR 1蛋白将被 分离的并且其区域对于转录激活是重要的， 甘油生长的调节，低聚化，葡萄糖控制， 和诱导的小的将被识别。 蛋白质， 结合或介导ADR 1作用将进行生物化学研究， 遗传的 ADR 1激活所需的SAF 1 -3基因 将对ADH 2转录进行克隆和测序。 的作用 CCR 1和SCH 9蛋白激酶，其作用独立于ADR 1 还将确定控制ADH 2。

The goal of this research is to elucidate the mechanisms of eucaryotic gene regulation. The system chosen for study is the yeast transcriptional activator ADR1 in its control of glucose repressible alcohol dehydrogenase (ADH2). Particular emphasis will be placed on defining the mechanisms by which ADR1 is regulated, the functional regions of ADR1, the factors which mediate or influence ADR1 function, and the means by which these factors and ADR control ADH2 transcription. A combination of biochemical, recombinant DNA, and genetic analyses will be used. The in vivo phosphorylation site of ADR1 will be identified and the role of cAMP-dependent (cAPK) and -independent protein kinases in ADR1 regulation will be ascertained. The mechanisms by which ADR1 will be identified. The ADR1 protein will be isolated and its regions important to transcriptional activation, regulation of glycerol growth, oligomerization, glucose control, and induction of petites will be identified. The proteins which bind to or mediate ADR1 action will be studied biochemically and genetically. The SAF1-3 genes required for ADR1 activation of ADH2 transcription will be cloned and sequenced. The role of the CCR1 and SCH9 protein kinases which act independently of ADR1 controlling ADH2 will also be determined.