Characterization and Inhibition of Plant and Bacterial Protoporphyrinogen Oxidase

原卟啉原氧化酶，血红素和叶绿素中的酶 合成，正在植物系统中表征。 初始 实验集中在二苯醚 除草剂，最近被鉴定为这种酶的抑制剂， 植物提取物对原卟啉原氧化的影响 细胞器和纯化的酶。 抑制 这些除草剂对原卟啉原的氧化作用 在大麦的叶绿体和线粒体中进行了检查， 对二苯醚和大豆敏感，大豆似乎 抵抗 血液红色素和叶片绿色色素的含量分别为 在结构上相互关联超过100 年前 它们都含有称为卟啉的有机部分。 尽管我们对动物合成途径的了解 相当广泛，我们对植物（和细菌）如何制造 卟啉仍然相当有限。 该项目的重点是一个 植物酶的研究是一个重要而又困难的课题 通路 一个新的工具已成为研究中的一个 抑制叶绿素形成的除草剂。 这 有望在植物的一个有点棘手的领域取得新的进展 生物学

Protoporphyrinogen oxidase, an enzyme in heme and chlorophyll synthesis, is being characterized in plant systems. The initial experiments focus on the mechanism by which diphenyl ether herbicides, recently identified as inhibitors of this enzyme, affect protoporphyrinogen oxidation by extracts of plant organelles and by the purified enzyme. Inhibition of protoporphyrinogen oxidation by these herbicides is being examined in chloroplasts and mitochondria from barley, which is sensitive to diphenyl ethers, and soybean, which appears to be resistant. The red pigment of blood and the green pigment of leaves were shown to be related to each other structurally more than 100 years ago. They both contain organic portions called porphyrins. Although our knowledge of the synthetic pathways in animals is quite extensive, our knowledge of how plants (and bacteria) make porphyrins is still quite limited. This project focuses on a very important but difficult to study enzyme of the plant pathway. A new tool has become available for the study in a herbicide which inhibits the formation ofchlorophyll. This promises new advances in a somewhat intractable area of plant biology.