Characterization of Synaptonemal Complexes and RecombinationNodules From Lilium longiflorum

相关证据表明，联会复合体（SC）和 重组结节（RN）参与染色体联会， 基因重组然而，人们对它的成分知之甚少。 可能有助于阐明其确切功能的SC和RNS。 本研究拟从百合属植物中分离SC和RNs longiflorum，并通过以下方法表征来自分离的SC/RN的蛋白质： 凝胶电泳 分离的SC/RN也将被注射到小鼠中。 来引发抗体 将制备杂交瘤并筛选， 分离的SC/RN的免疫荧光。 的单克隆抗体 然后将使用（1）识别Western中的SC/RN蛋白 印迹，（2）检查发育和组织特异性表达 SC/RN蛋白，（3）确定SC/RN蛋白的交叉反应性， 其他植物和动物SC和RN的抗体，以及（4）确定 在四个分阶段期间观察到的结核之间的关系 减数分裂前期I（细线期、偶线期、粗线期和双线期）。 这些抗体随后可用于亲和层析和/或免疫层析。 免疫沉淀以大量分离SC/RN蛋白， 确定它们的功能并识别编码它们的基因。 这项工作有可能导致我们的研究取得真实的突破。 理解SC和RN的功能。

Correlative evidence suggests that synaptonemal complexes (SCs) and recombination nodules (RNs) are involved in chromosome synapsis and genetic recombination. However, little is known about the constituents of SCs and RNS that might help elucidate their precise funtion(s). It is proposed to isolate SCs and RNs from the plant species Lilium longiflorum and to characterize the proteins from isolated SCs/RNs by gel electrophoresis. Isolated SCs/RNs will also be injected into mice to elicit antibodies. Hybridomas will be made and screened against isolated SCs/RNs by immunofluorescence. The monoclonal antibodies identified will then be used (1) to identify SC/RN proteins in Western blots, (2) to examine developmental and tissue-specific expression of the SC/RN proteins, (3) to determine the cross-reactivity of the antibodies to other plant and animal SCs and RNs, and (4) to determine the relationship between nodules observed during four of the substages of meiotic prophase I (leptotene, zygotene, pachytene, and diplotene). These antibodies later can be used for affinity chromatography and/or immunoprecipitation to isolate SC/RN proteins in large quantities to determine their functions and to identify the genes coding for them. This work has the potential to lead to a real break-through in our understanding of the function(s) of SCs and RNs.