US-UK Collaborative Research: Oxygen Consumption of Cells and Tissues

该奖项将支持博士之间的合作研究。 约翰盖纳，弗吉尼亚大学和博士P.A.阿伦德尔，ICI 制药公司，麦克尔斯菲尔德，柴郡，英格兰。 的 拟议研究的目的是调查 细胞和组织中的氧消耗。 从活细胞中生产产品， 微生物或动物，强烈依赖于全面的知识， 这类细胞的新陈代谢。 盖纳博士目前 弗吉尼亚大学的研究主要关注 与影响次级代谢产物产生的因素 从固定化的微生物细胞。 目前还在开展工作， 确定自由和固定动物的代谢需求 细胞，特别是关于氧消耗。 已经发现，每个细胞的耗氧速率 对于动物和微生物细胞来说都是非常相似的， 依赖于细胞的密度--随着 密度增加。 下一步是扩展这些结果 到非常高的细胞密度 这是可以做到的，在这种情况下， 动物细胞，通过研究氧气消耗， 组织中 ICI的药物研究中心位于 英国曼彻斯特有一个活跃的跨学科的 参与耗氧量基础研究的小组 和组织的代谢需要。 在为期四个月的研究中， 留在英国，盖纳博士建议进行联合 研究在ICI研究中心，这将涉及两个 影响组织耗氧量因素的研究 并将这些结果与之前的数据进行比较， 单个细胞。盖纳医生对 在氧气向细胞和生命系统的质量传递中。 ICI的代谢功能障碍团队拥有一流的设施， 并探讨了氧气消耗的问题， 从一个反应控制的机制。 的 结合两种观点，沿着设施 为获取数据所必需的，应相互补充 以及允许更完整地描述整个 耗氧过程

This award will support collaborative research between Dr. John Gainer, University of Virginia and Dr. P.A. Arundel, ICI Pharmaceuticals, Macclesfield, Cheshire, England. The objective of the proposed research is an investigation of oxygen consumption in cells and tissues. The manufacture of products from living cells, whether microbial or animal, depends strongly on a thorough knowledge of the metabolism of such cells. Dr. Gainer's current research at the University of Virginia is concerned primarily with factors affecting the production of secondary metabolites from immobilized microbial cells. Work is also ongoing to define the metabolic needs of free and immobilized animal cells, in particular with respect to the oxygen consumption. It has been found that the oxygen consumption rate per cell is quite similar for both animal and microbial cells, and is dependent on the density of the cells--decreasing as the density increases. The next step is to extend these results to very high cell densities. This can be done, in the case of animal cells, by studying the oxygen consumption of tissues. ICI, at their Pharmaceuticals Research Center near Manchester , England, has an active, interdisciplinary group involved in basic research concerning oxygen consumption and metabolic needs of tissues. During a four month research stay in England, Dr. Gainer proposes to carry out joint studies at the ICI Research Center, which will involve both a study of the factors affecting oxygen consumption in tissues and a comparison of those results to previous data for individual cells. Dr. Gainer has had considerable experience in the mass transfer of oxygen to cells and living systems. The Metabolic Dysfunction Team at ICI has excellent facilities, and has approached the problem of oxygen consumption and metabolism from a more reaction-controlled mechanism. The combination of the two viewpoints, along with the facilities necessary for obtaining data, should complement each other well and allow for a more complete description of the entire oxygen consumption process.