Expression of Bacterial Hemoglobin in Streptomyces to Enhance Antibiotic Production

氧限制是抗生素的主要障碍 利用好氧细菌的发酵 链霉菌 目前增加氧转移的方法 包括改进的生物反应器设计， 改良培养基。 一个新的方法来解决这个问题， 氧转移是菌株的发展， 更有效地从介质中获得氧气。 当前 建议的目的是发展一个 一种转基因菌株， 血红蛋白样蛋白 这种蛋白质最初是 发现于需氧细菌Vitrebella， 使细胞能够在缺氧环境中存活 环境 当Vitrecha血红蛋白在 大肠杆菌，重组细胞， 表现出更高的生长和呼吸速率， 到更高的细胞密度。 编码这种蛋白质的基因 将被引入天蓝色链霉菌， 产生抗生素放线菌紫素。 的重组细胞 然后将对其进行生长和呼吸测试 性质和放线菌红素生产。 示范 提高生长、呼吸和/或放线菌紫素产量 将产生广泛的影响， 抗生素发酵，但对于任何生物过程系统， 涉及有氧细胞培养。

Oxygen limitation is a major obstacle in antibiotic fermentations that utilize the aerobic bacteria Streptomyces. Current methods to increase oxygen transfer to Streptomyces include improved bioreactor design and modified culture media. A novel approach to the problem of oxygen transfer is the development of strains that can obtain oxygen from the medium more efficiently. The current proposal is directed towards the development of a genetically modified strain that expresses a bacterial hemoglobin-like protein. This protein was originally discovered in the aerobic bacteria Vitreoscilla, and apparently enables the cells to survive in a oxygen-poor environment. When Vitreoscilla hemoglobin was expressed in the bacteria Escherichia coli, the recombinant cells demonstrated higher growth and respiration rates, and grew to higher cell densities. The gene encoding this protein will be introduced into Streptomyces coelicolor, which produces the antibiotic actinorhodin. The recombinant cells will then be tested for their growth and respiratory properties, and actinorhodin production. The demonstration of enhanced growth, respiration, and/or actinorhodin yields in this system will have broad implications not only for antibiotic fermentations but for any bioprocess system that involves aerobic cell culture.