Characterization of Ubiquitin Pathway in Higher Plants

蛋白质的翻译后修饰调节 重要的生理和生化过程。 一个这样 修饰是蛋白质的共价连接 泛素。 只有一小部分泛素的作用- 蛋白质缀合已经在真核生物中得到表征。 泛素的最好的研究角色是作为一个可重复使用的标签 标记蛋白质以进行蛋白质水解。 在植物中， 被发现附着在感光体光敏色素和外套上 烟草花叶病毒的蛋白质。 这样做的目的 研究的目的是探索泛素在 高等植物的生理过程。 生化 分析检查了泛素的亚细胞定位， 蛋白质结合物。 分子分析表明， 高等植物中的泛素基因表达涉及1） 泛素剩余成员的分离 拟南芥的延伸基因家族，2）确定 不同成员表达的调节 泛素延伸基因家族和3）定义的 泛素延伸基因的启动子区， 缺失分析和定点突变， 引入植物。 泛素是一种广泛存在于植物中的蛋白质， 动物、酵母和细菌细胞。 这是一个最 已知高度保守的蛋白质，只有三个氨基酸 人类和高等植物蛋白质的区别。 它 在细胞中扮演着许多重要的生物学角色， 最充分的特点是它的作用， 蛋白质，尤其是异常蛋白质。 泛素调控的研究进展 路径是基本的。 因此，这些研究的目的 是提供有关监管的基本信息， 泛素途径及其基因调控 高等植物。*

Post-translational modifications of proteins regulate important physiological and biochemical processes. One such modification is the covalent attachment of the protein ubiquitin. Only a small subset of the roles of ubiquitin- protein conjugation has been characterized in eukaryotes. Ubiquitin's most well-studied role is as a reusable tag marking proteins for proteolysis. In plants, it has been found attached to the photoreceptor phytochrome and the coat protein of Tobacco Mosaic Virus. The purpose of this research is to explore the function of ubiquitin in physiological processes in higher plants. The biochemical analysis examines the subcellular localization of ubiquitin- protein conjugates in Arabidopsis. The molecular analyses of ubiquitin gene expression in higher plants involve 1) isolation of the remaining member(s) of the ubiquitin extension gene family from Arabidopsis, 2) determination of the regulation of expression of different members of the ubiquitin extension gene family and 3) definition of the promoter regions for the ubiquitin extension genes by deletion analysis and site-directed mutagenesis and re- introduction into plants. Ubiquitin is a protein that is widely distributed in plant, animal, yeast and bacterial cells. It is one of the most highly conserved proteins known, having only three amino acid differences between the human and higher plant protein. It serves a number of important biological roles in the cell, the most fully characterized being its role in marking proteins for breakdown, especially abnormal proteins. Current understanding of the regulation of the ubiquitin pathway is rudimentary. Therefore, the goal of these studies is to provide fundamental information on the regulation of the ubiquitin pathway and the regulation of their genes in higher plants.***