Small Grants for Exploratory Research (SGER): Preparative- Scale Isoelectric Focusing in Open Channels Using Thermally-Engendered pH Gradients

这笔赠款是在工程和分析化学领域以及色谱学分领域。洛克穆勒教授将研究一种连续的、开放通道的等电聚焦系统的设计和应用，该系统将使用热产生的pH梯度来分离大尺寸的两性离子。这项研究的目标是开发间歇式和连续式等电聚焦系统，其分离能力将来自热产生的pH梯度。这项研究的长期影响将是开发简单、制备的等电聚焦仪器，而不是现有的分析仪器，能够分解和有效地分离大蛋白质和整个细胞。

This grant is in the areas of engineering and analytical chemistry and in the subarea of chromatography. Professor Lochmuller will study the design and application of a continuous, open-channel, isoelectric focusing system that will separate ampholytes of broad size scale using thermally produced pH gradients. This research will aim to develop both batch and continuous isoelectric focusing systems whose separating capabilities will arise from thermally produced pH gradients. The long- term impact of this research will be in the development of simple, preparative isoelectric focusing instruments, as opposed to existing analytical instruments, that are capable of resolving and efficiently separating both large proteins and whole cells.