Laser Ablation Time-of-Flight Mass Spectrometry of DNA

这项探索性研究补助金是在一般领域， 分析和表面化学以及质量子领域 光谱法 亚利桑那州立大学的彼得威廉姆斯教授 大学将研究分子离子物种的生产 从冷冻的含水DNA基质的激光挥发。 目标是尽量减少DNA的碎片， 保持生物分子量分布 分子。 发展一种适当的方法， 将产生用于测序生物分子的光谱法。 通过使用新的能量沉积机制， 的吸收率和相变热力学 矩阵用于保持结构。 的建设 飞行时间质量高分辨率飞行管 光谱分析将使人们能够立即了解 激光能量沉积参数对最小化 碎片化

This Grant for Exploratory Research is in the general area of analytical and surface chemistry and in the subfield of mass spectrometry. Professor Peter Williams of Arizona State University will study the production of molecular ion species from laser volatilization of frozen aqueous matrices of DNA. The goal is to minimize the fragmentation of the DNA and preserve the molecular weight distribution of the biological molecule. The development of a suitable means to use mass spectrometry to sequence biological molecules will result. Via the use of a novel energy deposition mechanism, both the absorptivity and the phase transformation thermodynamics of the matrix are used to preserve structure. The construction of a high resolution flight tube for time of flight mass spectrometry will allow immediate understanding of the role of laser energy deposition parameters on the minimization of fragmentation.