Lineage Analysis of Zebrafish Neurodevelopment

研究人员将确定在脊椎动物神经元发育过程中，细胞谱系在细胞命运规范中所起的作用。他们将研究斑马鱼的早期胚胎，在那里他们可以标记单个细胞，移植它们，并跟踪它们在活胚胎中的发育。他们之前已经证明，原肠细胞产生的克隆仅限于单一组织，但如果这些细胞被移植，它们就会产生新的细胞系。因此，早期细胞可能表现出决定其早期行为的位置特异性发育程序，但不限制其潜力。为了进一步了解细胞谱系的发育作用，研究人员将在体内研究通常由单个标记细胞产生的谱系，学习各种细胞类型（包括已识别的神经元）的分裂、运动模式和谱系关系。为了研究细胞的功能和环境提供的线索，他们将检查胚胎之间单独移植的细胞的谱系和命运。

The investigators will determine what role cell lineage plays in the specification of cell fate during vertebrate neuronal development. They will study the early embryo of the zebrafish, in which they can mark individual cells, transplant them, and follow them as they develop in living embryos. They have shown previously that gastrula cells generate clones restricted to single tissues, yet if the cells are transplanted, they produce new lineages. The early cells, therefore, may express position- specific developmental programs that determine their early behavior, but do not restrict their potential. To further our understanding of the developmental roles of cell lineage, the investigators will study in vivo the lineages that normally arise from singly marked cells, learning the patterns of division, movements, and lineage relationships of a variety of cell types, including identified neurons. To investigate cellular commitments and clues provided by the environment, they will examine lineages and fates of cells transplanted individually between embryos.