Whole-Brain Functional Imaging and Analysis of Zebrafish Sleep
斑马鱼睡眠的全脑功能成像和分析
基本信息
- 批准号:10772327
- 负责人:
- 金额:$ 16.83万
- 依托单位:
- 依托单位国家:美国
- 项目类别:
- 财政年份:2022
- 资助国家:美国
- 起止时间:2022-05-15 至 2025-04-30
- 项目状态:未结题
- 来源:
- 关键词:AnatomyAnimalsArousalBehaviorBehavioralBindingBiologicalBiologyBrainBrain imagingCalciumCellsCircadian RhythmsClassificationComplementComputing MethodologiesCustomDataDrosophila genusExperimental DesignsFoundationsFunctional ImagingFunctional disorderGeneticGoalsHeadHourImageLabelLightLightingLinkMammalsMapsMathematicsMediatingMethodsMicroscopyModelingMolecularMonitorNeuronsNeurosciencesNeurotransmittersOutcomePathway interactionsPatternPhysiologicalPopulationPreparationProcessRegulationResolutionRodentSepharoseSleepSleep DeprivationSleep DisordersSleep Disorders TherapySleep disturbancesSocietiesStatistical MethodsSystemTailTechniquesTestingTheoretical modelTimeWakefulnessWorkZebrafishcalcium indicatorcircadiancost estimatedata analysis pipelineimage processingimaging platformimprovedinsightlarge datasetsmathematical analysismathematical modelmodel organismmolecular markerneuralneuronal circuitryneuropsychiatric disorderoptogeneticspharmacologicpoor sleepresponsescale upsleep behaviorsleep regulationtheoriestooltwo-photon
项目摘要
ABSTRACT
Sleep occupies a third of our lives and sleep-related ailments cost an estimated $100 billion per year, yet the
mechanisms governing its regulation remain poorly understood. Despite the substantial progress that has been
made in the discovery and understanding of specific sleep-promoting and wake-promoting neuronal and
molecular pathways, what is missing is an integrated understanding of how these mechanisms work together in
the brain to regulate sleep and wake as whole-brain behavioral states. Toward this goal, we propose a
conceptually simple yet powerful approach: record the activity of every neuron in the brain during normal sleep
and wake states, and in response to perturbations that induce these states, then apply mathematical analysis
and modeling to uncover fundamental principles that underlie sleep. The main goals of this exploratory project
are to develop and validate imaging, analysis, and modeling tools that will serve as a foundation for a subsequent
larger-scale application that will comprehensively identify and characterize sleep-regulating circuits, and
generate models to explain the neuronal circuit principles that underlie sleep. We will use the small and
transparent larval zebrafish, a vertebrate model with well-characterized sleep behavior whose regulation is
conserved with that of mammals. Using this model and our custom-developed two-photon selective plane
illumination microscopy (2P-SPIM) platform, we will perform whole-brain recordings of neuronal activity with
cellular-resolution during both natural and induced sleep and wake states. We will then apply mathematical tools
to extract insights from these whole-brain recordings to identify the neural substrates that underlie sleep. Our
analysis will allow us to both test existing models of sleep regulation and to propose new models based on our
data. This project will be the first to achieve comprehensive observation and analysis of vertebrate sleep at such
scale and resolution. The unique insights gained from these studies will pave the way toward a more complete
understanding of the neuronal mechanisms that underlie sleep, whose dysfunction imposes a significant burden
on society.
摘要
项目成果
期刊论文数量(0)
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GEOFFREY J GOODHILL其他文献
GEOFFREY J GOODHILL的其他文献
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{{ truncateString('GEOFFREY J GOODHILL', 18)}}的其他基金
Whole-Brain Functional Imaging and Analysis of Zebrafish Sleep
斑马鱼睡眠的全脑功能成像和分析
- 批准号:
10430595 - 财政年份:2022
- 资助金额:
$ 16.83万 - 项目类别:
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