U.S.-UK Cooperative Research: Regulation of the Plasma Membrane and Tonoplast H+-ATPases in Plants Exposed to NaC1

该奖项将支持普渡大学园艺系的保罗·长谷川博士和普度大学园艺系的保罗·长谷川博士之间的合作研究。T. J. Flowers和A. R. Yeo，英国苏塞克斯大学生物科学学院。本课题的目的是研究质膜和细胞质atp酶对盐胁迫的响应。盐分是限制农业生产的一个主要因素，因为它降低了耕地的产量。植物的耐盐性涉及细胞学和解剖组织各层次机制的整合。至关重要的机制是那些调节离子积累速率和介导高离子积累效应的机制，同时促进足够的溶质吸收，以便在新的渗透环境中生长所必需的渗透调节。我们有理由设想，质膜和质质体在调节溶质积累和区隔中起着中心作用。然而，实验结果尚未明确质膜和质质体在质膜中的生理生化作用。该项目将重点关注质膜和细胞质H+ -易位atp酶，因为它们在产生跨膜的H+电化学梯度中起着核心作用，这促进了跨膜溶质进入细胞。该项目的目的是提供证据，证明这些氢离子泵在盐征收制度期间调节溶质进口到工厂的重要性。该项目将受益于美国和英国研究人员在盐胁迫分子生物学和生理学方面的互补专业知识。这项研究的结果可能对农业具有重要的经济意义。

This award will support collaborative research between Dr. Paul Hasegawa, Department of Horticulture, Purdue University, and Drs. T. J. Flowers and A. R. Yeo, School of Biological Sciences, University of Sussex, England. The objective of the proposed project is to study the response of the plasma membrane and tonoplast ATPase to salt stress. Salinity is a major factor limiting agricultural production, since it reduces yields in land under cultivation. Salt tolerance in plants involves the integration of mechanisms at all levels of cytological and anatomical organization. Mechanisms of critical importance are those which function to regulate the rate of ion accumulation and mediate the effects of high ion accumulation, yet promote sufficient solute uptake for osmotic adjustment necessary for growth in the new osmotic environment. It is reasonable to envisage that the plasma membrane and the tonoplast have central roles in regulated solute accumulation and compartmentation. However, experimental results have not defined the physi- ological or biochemical roles of the plasma membrane and tonoplast in plasma membrane. This project will focus on the plasma membrane and tonoplast H+ - translocating ATPases because of their central roles in the generation of the H+ electrochemical gradient across the respective membranes, which mediates facilitated transmembrane solute import into the cell. The objective of this project is to provide evidence regarding the significance of these H+ - pumps in the regulation of solute import into the plant during regimes of salt imposition. This project will benefit from the complementary expertise of the US and British investigators in molecular biology and physiology of salt stress. The results of this research may have important economic implications for agriculture.