Replication Strategy and Control of a Plant Rhabdovirus

该项目有两个主要目标，旨在增加我们对植物横纹肌病毒（sonchus yellow net virus， SYNV）复制的了解。这些目标是：继续正在进行的烟草原生质体中SYNV复制的研究；并开发一个体外系统来研究SYNV基因组的转录和复制。原生质体实验将有助于更详细地了解病毒复制周期中发生的事件，并将允许分析宿主在此过程中可能发挥的作用。体外系统将产生与SYNV基因组产物的转录、复制和封装调控相关的信息。因此，该项目将为了解负链病毒的生命周期及其基因产物的治疗应用提供具有广泛生物学意义的信息。

This project has two primary objectives designed to increase our understanding of the replication of a plant rhabdovirus, sonchus yellow net virus (SYNV). These objectives are: to continue ongoing studies of SYNV replication in infected tobacco protoplasts; and to develop an in vitro system for investigating transcription and replication of the SYNV genome. The protoplast experiments will contribute to a more detailed understanding of events occurring during the replication cycle of the virus and will permit analysis of the roles the host may play in this process. The in vitro system will yeild information relevant to the regulation of transcription, replication and encapsidation of the SYNV genome products. Thus, this project will provide information of broad biological significance for understanding the life cycles of negative strand viruses and applications of their gene products for therapeutic purposes.