Improvements in the Physical-Analytical Sequence. Thermodynamic and Chromatographic Studies on Biological Systems

在乔治亚州立大学的物理分析序列中，溶液现象和热力学分析的研究正在取得进展，高压液相色谱（HPLC）现在是色谱领域的主要技术。连接到个人电脑的自动热滴定和pH滴定系统正在被学生们用来探索，在生物学背景下，质子转移和金属离子结合的热力学和分析方面。可以在小体积上进行数百甚至数千个数据点的滴定，从而允许使用昂贵的生物样品和比目前多十倍的实验性能。学生们正在使用高效液相色谱法进行旋光活性成分的分离，建立超声DNA样品和直链淀粉混合物的分子量分布，探索色谱法的分析能力，并确定止痛药中特定成分的检测限。几个生物系统的大量数据将与最新的数学处理相结合，以提供所研究的每个系统的物理参数及其相关误差，并在某些情况下测试化学模型的有效性。该机构为该项目提供资金，金额与NSF基金相等。

In the physical-analytical sequence at Georgia State University, improvements are being made in studies on solution phenomena and thermodynamic analyses, and high pressure liquid chromatography (HPLC) is now the primary technique in the chromatography area. Automated thermal and pH titration systems connected to personal computers are being used by students to explore, in a biological context, the thermodynamic and analytical aspects of proton transfer and metal ion binding. Titrations with hundreds or even thousands of data points can be performed on small volumes, thus allowing use of costly biological samples and performance of up to ten times more experiments than at present. Students are using HPLC to perform separations of optically active components, establish the molecular weight distribution for sonicated DNA samples and amylose mixtures, explore the analytical capabilities of chromatography and determine the detection limits for a particular component in an analgesic. Extensive data for several biological systems will be combined with the latest mathematical treatments to provide physical parameters and their associated errors for each system studied, and in some cases test the validity of chemical models. The institution is contributing to this project and amount equal to the NSF funds.