Development and Validation of Analytical and Physical Testing Methods to Evaluate the Irradiation History of Animal Feeds

评估动物饲料辐照历史的分析和物理测试方法的开发和验证

• 批准号: 9279603
• 负责人: J. Scott Smith
• 金额: $ 9.9万
• 依托单位: KANSAS STATE UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2016
• 资助国家: 美国
• 起止时间: 2016-09-01 至 2021-08-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/9279603
• 关键词: Development; Validation; Analytical; Physical; Testing

Abstract Over the last decade, numerous cases of illness and death in dogs, which consumed imported irradiated chicken jerky treats, and in parallel outbreak of neurological disorders and deaths in cats in Australia, which fed irradiated food, raised attention to the safety of the irradiated pet food products. Although the actual cause is still unknown, determining the total irradiation dose is invaluable to ensure the products are processed within the safe limits. Among the methods established for the detection of irradiated foods, analysis of 2-alkylcyclobutanones (2-ACBs) with GC-MS and analysis of cellulosic and carbonate radicals with electron paramagnetic resonance (EPR) spectroscopy serve as the most promising approaches to determine the irradiation history in pet food products. The goal of the proposed research is to develop GC-MS and EPR techniques coupled to quantify the formation of specific irradiation markers and to determine the total amount of irradiation treatment in pet foods and treats. We will carry out the project in two parts: the first three years will be dedicated to development and validation of testing methods; the last two years will be the comparison and field-testing of the validated protocols for evaluation of the irradiation history in pet food products. In the first part of the project pet food products, including chicken jerky treats, sweet potato treats, dog chews (beer, lamb), bones, hoofs and pig ears, will be irradiated from 0.1-75 kGy with either a Cesium-137 source and electron beam (e-beam). The formation and stability of various 2-ACBs (i.e., 2-DCB, 2-TCB, 2-TDCB) will be evaluated using GC-MS techniques based on our previous works in collaboration with Vet-LIRN and USDA labs. In parallel, formation and stability of carbonic and cellulosic radicals will be evaluated by using EPR analysis in both low mobility and high mobility environments. Complementary to EPR measurements, irradiation-specific DNA damage will also be investigated by using qPCR analysis. The analytical and physical methods will be validated for different product formulations with varying lipid composition, moisture content, presence of glycerin, vegetables, phytochemicals etc., and extraction methods, such as hexane extraction, solid phase extraction (SPE), solid phase microextraction (SPME), soxhlet extraction. The chemical and thermal stabilities of the specific irradiation markers will be analyzed over a temperature range (20-80 °C) and at least one year of storage at room temperature. In the second part of the project, validated protocols will be tested on commercial irradiated pet food products available in the market. Eventually, we will compare and contrast the results obtained from physical and analytical techniques. Overall, at the end of the project we will propose validated and tested protocols, which can be used as standardized techniques to quantify the irradiation doses in various pet food products.

抽象的 过去十年中，因食用进口辐照鸡肉干而导致狗患病和死亡的案例不胜枚举 治疗，同时在澳大利亚，喂食辐照食物的猫也爆发了神经系统疾病和死亡，提高了 关注辐照宠物食品的安全性。尽管实际原因尚不清楚，但确定总 辐照剂量对于确保产品在安全限度内加工至关重要。已建立的方法中 用于检测辐照食品、使用 GC-MS 分析 2-烷基环丁酮 (2-ACB) 以及纤维素分析 碳酸盐自由基与电子顺磁共振（EPR）光谱是最有前途的方法 确定宠物食品中的辐照历史。拟议研究的目标是开发 GC-MS 和 EPR 结合技术来量化特定辐射标记的形成并确定辐射总量 宠物食品和零食中的辐照处理。我们将分两部分实施该项目：前三年将 致力于测试方法的开发和验证；过去两年将是比较和现场测试 用于评估宠物食品辐照历史的经过验证的协议。在该项目的第一部分宠物食品 产品包括鸡肉干、红薯、狗咬胶（啤酒、羊肉）、骨头、蹄子和猪耳朵。 使用铯 137 源和电子束（电子束）进行 0.1-75 kGy 的辐照。的形成与稳定性 将根据我们之前的工作，使用 GC-MS 技术评估各种 2-ACB（即 2-DCB、2-TCB、2-TDCB） 与 Vet-LIRN 和 USDA 实验室合作。同时，碳自由基和纤维素自由基的形成和稳定性 将通过在低流动性和高流动性环境中使用 EPR 分析进行评估。 EPR 的补充 测量，辐射特异性 DNA 损伤也将通过使用 qPCR 分析进行研究。分析和 物理方法将针对具有不同脂质成分、水分含量、 甘油、蔬菜、植物化学物质等的存在，以及提取方法，如己烷提取、固相提取 萃取（SPE）、固相微萃取（SPME）、索氏萃取。的化学和热稳定性 将在温度范围（20-80°C）和在室温下储存至少一年的情况下对特定辐照标记物进行分析 温度。在该项目的第二部分，将在商业辐照宠物食品上测试经过验证的协议 市场上有售的产品。最终，我们将比较和对比从物理和物理方面获得的结果 分析技术。总的来说，在项目结束时，我们将提出经过验证和测试的协议，可以使用 作为量化各种宠物食品中辐照剂量的标准化技术。