Enzyme Electrodes Based on Photoactivated Conducting Organic Polymers

导电有机聚合物是一类新的有机聚合物 低成本的轻质导电材料， 大量重要的实际应用。 这些 聚合物作为一种 制造基于酶的电化学生物传感器， 有独特的能力。 然而， 目前用于形成的电化学方法 这些聚合物在电极表面形成薄膜 对它们在酶中应用施加了严格的限制 个电极 一种形成导电聚合物的全新方法， 例如聚吡咯。 该方法 非常适合于酶电极的制造。 新 转导氧化还原酶反应的方法 将酶转化为电信号将由此产生 approach. 可行性研究将使用 黄嘌呤氧化酶和L-氨基酸氧化酶，以确定是否 它可以被固定到形成的导电聚合物膜中， 非电性过程。 的酶的活性 将检查它们的衬底。 这种新方法 将构成酶电极形成的基础 使用灵活的光沉积技术， 用于大规模生产导电聚合物。

Electrically conducting organic polymers are a new class of low-cost lightweight conducting materials that have a large number of important practical applications. These polymers are of particular interest as a means of fabricating enzyme-based electrochemical biosensors that have unique capabilities. However, several aspects of the electrochemical methods presently used for the formation of these polymers as thin films at electrode surfaces impose severe restrictions for their use in enzyme electrodes. A radically new method for forming conducting polymers, such as polypyrrole, has been developed. This method is highly suited to the fabrication of enzyme electrodes. New methods for transducing the reaction of an oxidoreductase enzyme into an electrical signal will arise from this approach. A feasibility study will be performed using xanthine oxidase and L-amino acid oxidase to determine if it can be immobilized into conducting polymer films formed by the non-electrical process. The activity of the enzymes toward their substrates will be examined. This new method will form the basis for the formation of enzyme electrodes using a flexible photodeposition technique, and can be used for the production of conducting polymers on a large scale.