Engineering Research Equipment Grant: HPLC

本高效液相色谱（HPLC）系统的主要目的是研究杂交瘤和四角瘤生产单克隆抗体（MAb）的稳定性。两个杂交瘤在悬浮和固定系统中长期连续培养的融合产物。采用优化的高效液相色谱法，在长期连续培养过程中监测亲本杂交瘤和四方瘤抗体的数量和浓度。研究结果将与流式细胞术和代谢研究相结合，以阐明提高杂交瘤和四角瘤单克隆抗体生产稳定性的机制。

The primary purpose for this High Performance Liquid Chromatography (HPLC) system is an investigation of the stability of monoclonal antibody (MAb) production by hybridomas and quadromas, the fusion product of two hybridomas, during long-term continuous culture in suspension and immobilized systems. Using an optimized HPLC process, the number and concentrations of antibodies for both parent hybridomas, and the quadroma, will be monitored during long-term continuous culture. Results will be integrated with concurrent flow cytometry and metabolic studies in an effort to elucidate mechanisms for improving the stability of MAb production by hybridomas and quadromas.