Virus Expression in Plants

这个项目的目的是检测远程病毒 为了更好地了解植物的运动， 植物抗病毒性 在过去的几年里， 研究主要集中在病毒编码因子在 细胞间的运动 该提案侧重于东道国因素 花椰菜花叶病毒（CaMV）在植物中的运动 拟南芥属植物。 新技术将被用于跟踪 病毒移动包括整株原位杂交 程序和常驻报告基因的病毒反式激活。 这些技术将被用来检查不同的拟南芥 生态型和筛选拟南芥突变体与抗性 病毒感染和长距离病毒模式的变化 运动 编码运动性状的基因将利用图- 基于克隆或缺失消减杂交。 深入了解病毒在体内运动的机制 植物会提出防止这种运动的机制， 从而产生抗病毒植物。*//

The purpose of this project is to examine long-distance virus movement in plants in order to obtain a better understanding of plant virus resistance. In the past few years, virus movement studies have centered around the role of virus-encoded factors in cell to cell movement. This proposal focusses on host factors which are involved in cauliflower mosaic virus (CaMV) movement in Arabidopsis plants. New techniques that will be used to follow virus movement include a whole plant in situ hybridization procedure and virus transactivation of a resident reporter gene. These techniques will be used to examine different Arabidopsis ecotypes and screen for Arabidopsis mutants with resistance to virus infection and variation in patterns of long distance virus movement. Genes encoding movement traits will be cloned using map- based cloning or deletion subtraction hybridization. Insight into mechanisms involved in the movement of viruses in plants will suggest mechanisms for preventing such movement and thereby creating a virus resistant plant.***//