Molecular Basis of Growth in Plants: Probing the Role of Wall Polysaccharides and Their Metabolism by Wall Proteins

植物细胞伸长是一种代谢变化的表达，这种代谢变化改变了细胞壁内的承载键，使分子在内部压力的影响下发生位移。具体来说，该提案提出了这样一个假设，即特定的壁多糖结构被修改，从而影响了这些承重键。所使用的检测系统将是玉米胚芽菌。主要研究方向包括：1)建立定量评价多糖与纤维素结合的体外体系。该模型可以确定pH值对结合的影响程度，以及酶修饰结构后pH值的影响。2)该实验将扩展抗体是探索细胞壁代谢的有力探针的观察。应用一系列葡聚糖和葡聚糖酶抗体的结果将扩展到确定组织暴露于葡萄糖醛酸阿拉伯氧素抗体的后果。阿魏酸的行为和与这些分子相连的二阿魏酸的作用将被评估。3)(2)的一个重要组成部分将是应用一种新的酶机制，利用葡萄糖醛酸氧化酶（以前称为铁烷酶）修饰葡萄糖醛酸阿拉伯糖木聚糖以确定结构（特别是葡萄糖醛酸和阿魏酸成分）。第二个重点是基于观察到抗体的空间（定向）应用对其抑制生长的能力很重要。这一努力将有助于确定表皮在控制伸长中的作用。结构和代谢方面也将检查在分离的表皮细胞壁。//本实验将有助于阐明植物生长的分子机制

Plant cell elongation is an expression of metabolic changes that alter load bearing bonds within the wall to allow molecular displacement under the influence of internal pressures. Specifically, the proposal addresses the hypothesis that specific wall polysaccharide structures are modified in such a manner that such load bearing bonds are affected. The assay system utilized will be the Zea mays coleoptile. The major directions the proposal explores include: 1) The development of an in vitro system to quantitatively evaluate polysaccharide binding to cellulose. This model will allow a determination of the extent to which binding is affected by pH and the effect of pH after modification of structures by enzymes. 2) The experiments will extend the observation that antibodies are powerful probes to explore cell wall metabolism. The results derived from application of a family of glucan and glucanase antibodies will be extended to determine the consequences of exposure of tissue to glucuronoarabinoxylan antibodies. The behavior of ferulic acid and the role of diferulic acid linked to these molecules will be assessed. 3) An important component of (2) will be the application of a new enzyme mechanism, using glucuronoxylanase (previously called feraxanase), to modify glucuronoarabinoxylans to determine structure (especially glucuronic acid and ferulic acid components). A second emphasis is based on the observation that spatial (directional) application of antibodies is important in their capacity to suppress growth. This effort will help to determine the role of the epidermis in controlling elongation. Structural and metabolic aspects will also be examined in isolated epidermal cell walls. The experiments described in this proposal will help to elucidate the molecular mechanism of plant growth.//