Determination of the Mechanisms of Cell Adhesion to and Damage from Air-Liquid Interfaces

拟议工作的总体方法是 从根本上了解细胞损伤的机制， 与现象学/相关类型的 这是历史上用来解决这个问题的方法。 问题. 建议的方法要求 识别负责细胞的过程 通过初步的可视化研究。 理论 并进行相关性研究， 实际生物反应器操作以确认这些 机制解释了观察到的细胞损伤。 一旦 这些机制得到证实，计算机模拟 流体动力学事件，如气泡破裂，将 用于确定实际“强度”或“灵敏度” 细胞对水动力的反应。

The overall approach of the proposed work is to fundamentally understand the mechanisms of cell damage in contrast to the phenomenological/correlation type of approach which has been historically used to address this problem. The proposed approach requires the identification of the processes responsible for cell damage through initial visualization studies. Theoretical and correlational studies are then to be carried out in actual bioreactor operations to confirm that these mechanisms account for the observed cell damage. Once these mechanisms are confirmed, computer simulations of the hydrodynamic events, such as bubble rupture, will be used to determine the actual "strength" or "sensitivity" of cells to hydrodynamic forces.