Bio-Imaging Analyzer (Phosphorimager)

该提案涉及购买富士生物成像的请求 分析仪（Phosphorimager），一种用于检测和 放射性的定量用于各种生化 实验 该仪器的定量能力将 允许目前不可能的生化实验。主 该技术优于常规放射自显影的优点 使用X射线胶片的优点是线性响应，动态范围， 和灵敏度，以及减少曝光时间。 仪器 提供了实验的定量数据， 这种放射性同位素如32P、35S、14C和125I。 的工具 怀特黑德研究所目前还没有这种类型的产品。 放射性样品的定量将由 各种生物化学和分子生物学的用户群 实验细节见第8节。 这项技术涉及到使用 存储来自样品β的能量的存储荧光屏 粒子发射 该发射被仪器检测到， 存储在计算机硬盘上。 然后可以访问数据， 使用仪器提供的软件包进行分析。 一 一些定量生化实验目前还没有 可能是可行的，因为Phosphorimager是巨大的 上级于X线胶片放射自显影。

This proposal involves a request to purchase the Fuji Bio-Imaging Analyzer (Phosphorimager), a new device for the detection and quantitation of radioactivity used in a variety of biochemical experiments. The quantitative capabilities of this instrument will allow biochemical experiments not currently possible. The primary advantages of this technology over conventional autoradiography using x-ray film are an increased linear response, dynamic range, and sensitivity, as well as reduced exposure time. The instrument provides quantitative data from experiments involving the use of this radioisotopes such as 32P, 35S, 14C, and 125I. An instrument of this type is not currently available at the Whitehead Institute. The quantitation of radioactive samples will be carried out by the user groups in a variety of biochemical and molecular biology experiment detailed in Section 8. The technology involves the use of storage phosphor screens which store energy form sample beta particle emission. This emission is detected by the instrument and stored on a computer hard disk. The data can then be accessed and analyzed using a software package provided with the instrument. A number of quantitative biochemical experiments not currently possible will be feasible because the Phosphorimager is vastly superior to X-ray film autoradiography.