The Infrastructure of Mitochondrial Matrix Proteins

现在有许多令人信服的证据表明线粒体内的代谢途径是有组织的。这些途径包括Krebs TCA循环、脂肪酸氧化、氨基酸代谢和Krebs尿素循环。与分子组织有关的三个领域仍有待阐明，涉及特定蛋白质-蛋白质相互作用的化学性质，代谢产物中断的代谢后果，以及超分子复合体与线粒体整体形态的关系。本研究的具体目的是：a)确定柠檬酸合成酶与其膜结合蛋白(柠檬酸转运体？)相互作用的结合部位。以及与其反应伙伴苹果酸脱氢酶、丙酮酸脱氢酶复合体、硫解酶和乌头酸酶；b)分析线粒体中含有过氧化体柠檬酸合酶的酵母细胞的生长和代谢模式；以及c)确定从肾上腺线粒体内膜引入特征膜蛋白是否可以改变酵母线粒体的晶体形态。

There is now convincing evidence on many levels that metabolic pathways within the mitochondrion are organized. These pathways include the Krebs TCA cycle, fatty acid oxidation, amino acid metabolism, and the Krebs urea cycle. Three areas concerning molecular organization which are still to be elucidated concern the chemical nature of the specific protein-protein interactions, the metabolic consequences of disrupted metabolons, and the relation of the supramolecular complexes to the overall morphology of the mitochondrion. The specific aims of this research are to: a) determine the binding sites on citrate synthase that interact with its membrane binding protein (citrate transporter?) and with its reaction partners, malate dehydrogenase, pyruvate dehydrogenase complex, thiolase, and aconitase; b) analyze the growth and metabolic pattern of yeast cells containing peroxisomal citrate synthase in their mitochondria; and c) determine whether cristal morphology of yeast mitochondria can be changed by the introduction of a characteristic membrane protein from adrenal mitochondrial inner membranes.