Modulation of Active Oxygen During Pathogenesis of Pseudomonas Syringae

本项目将研究活性氧在 抑制植物病原菌的致病作用 假单胞菌 假设病原体 假单胞菌能够减少植物施加的氧化应激 在发病过程中，虽然过氧化氢酶同工酶的阿森纳将是 测试. 该项目有两个部分：1）克隆 过氧化氢酶同工酶的基因和突变体的构建 毒力研究; 2）确定 各种过氧化氢酶基因与HRP基因，似乎控制 细菌的致病性。 基因部分将是 由马里兰州大学的哈奇森博士进行， 生物化学和生理学研究将由博士进行。 Klotz在犹他州州立大学获得另一个奖项。 %%% 这项研究将有助于了解最初的 在植物与微生物相互作用过程中发生的事件， 提供了关于以下基本区别的新信息： 导致植物病害的微生物和那些不能导致植物病害的微生物。

This project will investigate the role of active oxygen in restricting pathogenesis by the plant pathogenic bacterium Pseudomonas syringae. The hypothesis that the pathogenic Pseudomonads are able to reduce plant-imposed oxidative stress during pathogenesis though an arsenal of catalase isozymes will be tested. There are two portions to the project: 1) cloning of the genes for the catalase isozymes and construction of mutants for virulence studies; and 2) determination of the interactions of the various catalase genes with the hrp genes that appear to control the pathogenicity of the bacterium. The genetic part will be carried out by Dr. Hutcheson at the University of Maryland, while biochemical and physiological studies will be carried out by Dr. Klotz at Utah State University under another award. %%% This research will contribute to the understanding of the initial events that occur during plant-microbe interactions, and should provide new information on the fundamental differences between microbes that causes plant disease and those which cannot.