Methods for tagging and mutating Arabidopsis genes with transposons.

该奖项的目的是利用玉米ac转座子元件，为拟南芥开发一种高效的转座子标签系统。Fedoroff博士和她的同事将通过创建含有转座子的T-DNA载体来优化检测序列未知的基因插入突变的能力，该载体可以(1)选择标记转座子已经切除的植物，(2)选择已经重新插入转座子的植物，以及(3)筛选转座子携带的标记基因的组织特异性激活的植物。他们还将开发使用转座子的方法来识别并在已知序列的基因中产生突变。利用转座子产生的突变提供了一种快速克隆基因的方法，即使在对基因序列一无所知的情况下，也可以使用该元件作为探针。通过插入转座子标签产生的突变类型应该有助于通过在体内创建启动子-报告基因融合来分析基因调控。这一奖项的结果应该会促进协调的拟南芥基因组研究项目的快速进展。

The objective of this award is to develop an efficient transposon tagging system for Arabidopsis thaliana, using the maize Ac transposable element. Dr. Fedoroff and her colleague will optimize the ability to detect insertion mutations in gene whose sequence is unknown by creating transposon-containing T-DNA vectors that make it possible to (1) select plants in which the tagging transposon has excised, (2) select plants in which the transposon has reinserted, and (3) screen plants for tissue-specific activation of a transposon-borne marker gene. They will also develop methods for using transposons to identify and create mutations in genes whose sequence is known. Mutations made with transposons provide a rapid means for cloning a gene, using the element as a probe, even when nothing is known about the gene's sequence. The types of mutations that will arise by insertion of the transposon tags should facilitate analysis of genes regulation by creating promoter- reporter gene fusions in vivo. The result of this award should facilitate rapid progress of the coordinated Arabidopsis genome research project.