Instrumentation for Undergraduate Biochemistry Laboratories

该项目的目标是使用单一的酶系统，二氢叶酸还原酶，作为新的非专业生物化学实验课程的各种实验的基础，并用于专业高级课程的现代化。利用克隆的基因作为一种酶的丰富来源，这种酶在癌症化学治疗、酶机制、基因扩增和分子进化中发挥重要作用，使学生能够学习广泛的技术，并为练习提供连续性。通过分光光度计、离心机、分馏器和电泳仪，学生可以学习蛋白质纯化、酶动力学、免疫印迹、紫外差分光光度法、质粒分离、限制性内切酶图谱和DNA测序。

The goal of this project is to use a single enzyme system, dihydrofolate reductase, as the basis for a variety of experiments in a new biochemistry laboratory course for non- majors, and in the modernization of an advanced course for majors. Using a cloned gene as an abundant source of an enzyme that plays an important role in cancer chemotherapy, enzyme mechanisms, gene amplification and molecular evolution allows students to learn a wide range of techniques and provides continuity to the exercises. The spectrophotometer, centrifuges, fractionator, and electrophoresis equipment allow students to learn protein purification, enzyme kinetics, immunoblotting, ultraviolet difference spectrophotometry, plasmid isolation, restriction mapping, and DNA sequencing.