T4 Phage dNTP Synthetase, a Multienzyme Complex for Dexyribonucleotide

这项研究的长期目标是：（1）了解DNA是如何 前体生物合成受到调控;（2）了解如何 影响DNA前体合成的调节机制控制着 DNA复制的速率和保真度;（3）了解 DNA前体扰动的代谢和遗传后果 生物合成;（4）有助于理解 所涉及的酶的作用机制。 拟议 研究涉及一种多酶复合物，在T4噬菌体中形成， 感染的大肠杆菌，它进行合成， 脱氧核糖核苷三磷酸（dNTPs）。 这是T4 dNTP 合成酶复合物 基因和生物化学的财富 对T4的了解使我们能够采取各种有力的方法， 了解这个系统中蛋白质之间的相互作用。 %%% DNA复制使用前体脱氧核糖核苷 三磷酸盐，专门用于该目的， 其他没什么。 除了复制，dNTPs还用于DNA 修复和重组;此外，细菌在细胞壁中使用dTTP 合成. 这种有限的代谢作用使其成为 DNA的合成可能是在特定的 前体形成的水平。 这项研究试图描述 dNTP合成是如何控制的，以及这些控制的程度 这些机制有助于控制DNA复制。

The long-range goals of this research are (1) to understand how DNA precursor biosynthesis is regulated; (2) to understand how regulatory mechanisms affecting DNA precursor synthesis control the rate and fidelity of DNA replication; (3) to understand the metabolic and genetic consequences of pertubations in DNA precursor biosynthesis; and (4) to contribute toward an understanding of mechanisms of action of the enzymes involved. The proposed research involves a multienzyme complex, formed in T4 phage- infected Escherichia coli, which carries out the synthesis of deoxyribonucleoside triphosphates (dNTPs). This is the T4 dNTP synthetase complex. The wealth of genetic and biochemical knowledge of T4 permits a variety of powerful approaches to understanding protein-protein interactions in this system. %%% DNA replication uses precursors, the deoxyribonucleoside triphosphates, which are specialized for that purpose and for little else. In addition to replication, dNTPs are used in DNA repair and in recombination; also, bacteria used dTTP in cell wall synthesis. This limited repertoire of metabolic role makes it possible for DNA synthesis to be regulated specifically at the level of precursor formation. This research attempts to describe how dNTP synthesis is controlled and the extend to which these mechanisms contribute toward the control of DNA replication.