Undergraduate Laboratory in Engineering Biology at Princeton University

开发了一套连贯的实验室实验， 展示了化学工程原理的应用 在生物技术领域。该程序用于本科 化学工程实验室课程提供一套核心的 实验，介绍化学工程专业的学生 生物技术，并为学生提供深入的接触， 新设立的工程生物学证书课程。 实验室实验围绕生产， 碱性磷酸酶的分离和纯化 使用E.大肠杆菌或芽孢杆菌 枯草杆菌。实验遵循的过程， 用于酶的商业生产。它们包括： 1)一个发酵实验，证明微生物的生长 和产物形成以及O2传质; 2） 提取实验，证明细胞裂解和膜 3）细胞收获实验，比较 超滤和离心，并强调放大 注意事项; 4）纯化实验，证明 色谱和电泳分离方法， 蛋白质的表征;和5）酶动力学 酶作用机理实验演示方法 通过产物抑制动力学的分析。

A coherent set of laboratory experiments is developed that demonstrates the application of chemical engineering principles in biotechnology. This program is used in the undergraduate Chemical Engineering laboratory course to provide a core set of experiments that introduces Chemical Engineering students to biotechnology, and provides in-depth exposure for students in the newly instituted Engineering Biology Certificate Program. The laboratory experiments center around the production, isolation and purification of alkaline phosphatases from bacterial fermentations using either E. coli or Bacillus subtilis. The experiments follow the processes that would be used in the commercial production of the enzyme. They include: 1) a fermentation experiment, demonstrating microbial growth and product formation as well as O2 mass transfer; 2) an extraction experiment, demonstrating cell lysis and membrane permeabilization; 3) a cell harvesting experiment, comparing ultrafiltration and centrifugation and emphasizing scale-up considerations; 4) a purification experiment, demonstrating chromatographic and electrophoretic methods for isolation and characterization of proteins; and 5) an enzyme kinetics experiment, demonstrating methods for testing enzyme mechanisms by analysis of the kinetics of product inhibition.