Detection of Human Pathogenic Viruses in Aquatic Environments Using the Polymerase Chain Reaction

该奖项旨在支持一项技术研究，以确定环境水域中存在的致病生物的存在、识别和数量（如果存在）。该技术的基础是确定不同目标病原体特有的遗传物质的性质，使用一种分析程序，放大目标生物体的核酸链，以便于识别和定量。该研究包括使用旋转膜超滤介质对病原体进行浓缩，随后使用聚合酶链反应程序对病原体进行检测。本研究解决了目前用于确定环境水微生物质量的程序的局限性。这些程序依赖于对替代微生物的分析，作为确定其存在意义的一步。本项目是美国国家科学基金探索性研究小额资助项目（92-01324）的延续，该项目的研究成果用于制定本项目的研究计划。

This is an award to support research on a technique to determine the presence, identity if present and numbers of pathogenic organisms, if present, in environmental waters. The technique is based on determination of the nature of the genetic material that is unique to the different target pathogens using an analytical procedure that amplifies the nucleic acid chain of the target organisms for ease of identification and quantification. The research involves concentration of the pathogens using a rotating membrane ultrafiltration medium and subsequent detection of the pathogens using the polymerase chain reaction procedure. This research addresses limitations of procedures currently used for determining the microbiological quality of an environmental water. These procedures depend on the analysis for surrogate microorganisms as a step toward establishing the significance of their presence. This project is a renewal of work initiated under NSF Small Grant for Exploratory Research No. 92-01324 results of which were utilized to develop the research plan for this project.