A Large Area Real-Time Imaging System for Screening DNA Libraries

这是SBIR第二阶段项目。 本研究的目的 是开发一种用于筛选DNA文库的成像系统。 基因组研究是世界上最活跃的研究领域之一， 今天的生物学，以及分析DNA文库的改进方法， 有用的人 研究计划是利用一种新的核 检测器，用于生成所述图像的分布的真实的时间图像， 含有DNA样品的转移膜上的放射性。 将图像直接投影到初级琼脂平板上。 在一期工程中， 原型已经开发使用两个正交阵列的 每一个耦合到位置敏感的 光电倍增管 在第二阶段项目中，研究将 专注于改进原型的性能和设计 增加面积的方法。 如果成功，新设备将 提高了识别的效率和精度， 基因隔离。

This is a SBIR Phase II project. The objective of this research is to develop an imaging system for screening DNA libraries. Genome research is one of the most active research areas in biology today, and an improved way to analyze DNA libraries will be useful. The research plan is to utilize a new nuclear detector to generate a real time image of the distribution of radioactivity on the transfer membrane containing DNA samples. The image will be directly projected onto the primary agar plate. In the Phase I project, a new large area imaging instrument prototype has been developed using two orthogonal arrays of scintillating fibers, each coupled to a position-sensitive photomultiplier tube. In the Phase II project, research will focus on improving the performance of the prototype and designing the ways of increasing area. If successful, the new device will improve the efficiency and precision of the identification and isolation of genes.