Regulation of Pyrimidine Biosynthesis and Cell Growth

本文将采用遗传和分子结合的方法研究黑腹果蝇初级基因表达的调控机制。潜在的假设是，存在一些反式调控基因，它们与初级基因相互作用，产生基因表达的发育程序。在初级基因5'末端的组织特异性控制区域将被更精确地定位和表征。将分离融合蛋白并用于产生针对野生型mod(r)1和mod(r)2蛋白的抗体。这些抗体将用于确定野生型蛋白的组织分布和细胞定位。此外，抗体将用于基因产物的表征和纯化。最后确定mod(r)1和mod(r)2的零表型和正常功能。具有缺陷和零表型的突变体将被分离并表征。cDNA将继续被分离和测序，以确定这些基因的生物学功能。了解基因是如何被调控的，从而使生物体的精确发育模式几乎一成不变地发生，对于我们洞察生命过程是至关重要的。

The regulatory mechanisms utilized for the control of expression of the rudimentary gene of Drosophila melanogaster will be examined with a combination of genetic and molecular approaches. The underlying hypothesis is that there exist a number of trans- acting regulatory genes which interact with the rudimentary gene to produce the developmental program of gene expression. The tissue- specific control regions within the 5'end of the rudimentary gene will be more precisely mapped and characterized. Fusion proteins will be isolated and used to generate antibodies to the wild-type mod(r)1 and mod(r)2 proteins. These antibodies will be used to determine the tissue distribution and the cellular localization of the wild-type proteins. Additionally, the antibodies will be used for the characterization and purification of the gene products. Lastly, the null phenotypes and normal functions of mod(r)1 and mod(r)2 will be determined. Mutants which have deficiencies and have a null phenotype will be isolated and characterized. cDNA's will continue to be isolated and sequenced in an effort to determine the biological function of these genes. %%% An understanding of the many ways in which genes are regulated so that the precise developmental pattern of a living organism almost invariably occurs is critical to our insight into life processes.