Genetic Regulation of Streptomyces Antibiotics

链霉菌以其合成大量次生代谢物(抗生素)的能力而闻名。除了它们的商业重要性外，链霉菌还因为其复杂的发育周期而令人感兴趣。在这个生命周期的过程中，抗生素的合成受到发育的调节。这项研究的目的是确定天蓝色链霉菌抗生素合成的全球遗传调控方面。这项计划中的研究是基于本实验室最近发现的一组基因，这些基因调节抗生素基因的表达。目标包括Absa、Abb B和Mia基因的遗传和分子特征；利用报告基因融合研究Absa、AbB和Mia的调节，以及调查这些基因全球调节抗生素合成的机制。对abs和mia基因的分子遗传学分析不仅有助于我们理解微生物发育基因调控，而且有助于生物技术。对天蓝色链霉菌调控的理解很可能解释其他链霉菌和放线菌中抗生素调控的大部分，这增加了合理操纵商业上重要的代谢物生产的可能性，包括许多不断被发现的新代谢物。

Streptomyces are well-known for their capacity to synthesize a vast repertoire of secondary metabolites (antibiotics). Besides their commercial importance, the Streptomyces are interesting for their complex developmental cycle. In the course of this life cycle, antibiotic synthesis is developmentally regulated. The objective of this research is to define aspects of the global genetic regulation of Streptomcymes coelicolor antibiotic synthesis. The research planned is based on a set of genes, recently discovered in this laboratory, which regulate the expression of antibiotic genes. The goals include genetic and molecular characterization of the absA, absB and mia genes; study of the regulation of absA, absB and mia using reporter gene fusions, and investigation of the mechanism by which these genes globally regulate antibiotic synthesis. %%% This molecular genetic analysis of the abs and mia genes promises to contribute not only to our understanding of microbial developmental gene regulation, but also to biotechnology. Understanding of S. coelicolor regulation is highly likely to explain much of antibiotic regulation in other Streptomycetes and Actinomycetes, and this increase the potential for rational manipulation of production of commercially important metabolites including the many new metabolites that are continuously being discovered.